Premium
Interleukin 5 Regulation of Peritoneal B‐Cell Proliferation and Antibody Secretion
Author(s) -
WETZEL G. D.
Publication year - 1990
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1990.tb02747.x
Subject(s) - antibody , secretion , b cell , biology , microbiology and biotechnology , b 1 cell , lipopolysaccharide , immunology , t cell , endocrinology , antigen presenting cell , immune system
The influence of recombinant interleukin 5 (rIL‐5) on murine peritoneal B‐cell proliferation and antibody secretion was examined. Larger, Low buoyant density peritoneal B cells proliferated better with rIL‐5 than the smaller resting B cells. This was also true for splenic B cells; however, comparison of the respective populations showed the large peritoneal B‐cell responses to be cells proliferated in response to rIL‐5 when lipopolysaccharide (LPS) was present. No detectable difference in the fraction of proliferating splenic B cells was seen in the presence of rIL‐5. These results are consistent with expression of IL‐5 receptors on about 70% of low‐density peritoneal B cells as determined by fluorescent staining with anti‐II‐5 receptor monoclonal antibody (MoAb). IL‐5 also enhanced spontaneous and mitogen‐driven IgM secretion by both peritoneal and splenic B lymphocytes; the increases exhibited by peritoneal B cells, however, were at least twice those exhibited by splenic B cells. Spontaneous and mitogen‐driven secretion of auto‐antibodies to bromelain‐treated mouse erythrocytes (BrMRBC) by peritoneal B cells were also increased by this interleukin. Furthermore, rIL‐5 enhanced peritoneal B‐cell plaque‐forming cell (PFC) responses to TNP‐LPS but not to TNP‐Ficoll. Both an anti‐IL‐5R MoAb and an anti‐IL‐5 MoAb blocked the rIL‐5‐induced enhancement of proliferation and auto‐antibody PFC responses. Hence, IL‐5 appears to be important for the regulation of proliferation and antibody secretion by many murine peritoneal B cells.