Premium
Mitogen‐Induced Interleukin 2 and Gamma Interferon Production by CD4 + and CD8 + Cells of Patients with Inflammatory Arthritides. A Comparison between Cells from Synovial Fluid and Peripheral Blood
Author(s) -
HOVDENES J.,
GAUDERNACK G.,
KVIEN T. K.,
HOVDENES A. B.,
EGELAND T.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb02467.x
Subject(s) - lymphokine , phytohaemagglutinin , cd8 , synovial fluid , microbiology and biotechnology , immunology , cytokine , interleukin 2 , peripheral blood mononuclear cell , interferon gamma , cytotoxic t cell , medicine , in vitro , biology , lymphocyte , antigen , pathology , biochemistry , alternative medicine , osteoarthritis
The purpose of this study was to investigate interleukin 2 (IL‐2) and gamma interferon (IFN‐γ) production by purified CD4 + and CD8 + cells isolated from peripheral blood (PB) and synovial fluid (SF) of patients with rheumatoid arthritis (RA) and other inflammatory arthritides (non‐RA). CD4 + and CD8 + cells were selected positively by immunomagnetic separation. Supernatants of unstimulated CD4 + and CD8 + cells from both compartments did not contain any delectable IL‐2 or IFN‐γ, while supernatants of CD4 + and CDS I cells stimulated with phytohaemagglutinin and irradiated Raji cells mostly contained both cytokines. In vitro stimulated SF CD4 + cells gave supernatants with significantly less IL‐2 than supernatants from PBCD4 + cells, while in vitro stimulated SF CD4 + ‐cell supernatants contained significantly more IFN‐γ. SF CD4 + ‐cell supernatants contained significantly more IL‐2 than the parallel CD8 + supernatants, while there was no significant difference with regard to IFN‐γ content. The pattern of differences between SF ‐and PB‐derived T cells was the same for the two groups of patients, but the SF CD4 + cells from RA patients produced significantly less IL‐2 than the corresponding cells from the non‐RA group. The difference between SF and PB T cells with regard to lymphokine production is probably related to various degrees of in vivo pre‐activation. The results do not indicate a major T‐cell deficiency in relation to lymphokine production in RA.