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Preferential Expression of V H 5 and V H 6 Immunoglobulin Genes in Early Human B‐Cell Ontogeny
Author(s) -
CUISINIER A.M.,
GUIGOU V.,
BOUBLI L.,
FOUGEREAU M.,
TONNELLE C.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb02455.x
Subject(s) - microbiology and biotechnology , gene , ontogeny , biology , antibody , messenger rna , fetus , northern blot , gene expression , genetics , pregnancy
Analysis of Ig transcripts by dot blot hybridization revealed that V H 5 and V H 6 RNA first occurred during the 7th week of gestation in human fetal liver. At the same time, a Cλ or Cλ‐like chain was also expressed, in the absence of detectable K chains. In adult B cells, V H 3 appears to be the dominant family expressed. Because V H 5 and V H 6 were located as the most proximal V genes to the J H cluster, the orderly expression of human V H genes seems to resemble the situation reported for the mouse.