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Recombinant Human Tumour Necrosis Factor β (Lymphotoxin) Lacks Chemotactic Activity for Human Peripheral Blood Neutrophils, Monocytes, and T Cells
Author(s) -
MROWIETZ U.,
TERNOWITZ T.,
SCHRÖDER J.M.,
CHRISTOPHERS E.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01224.x
Subject(s) - lymphotoxin , recombinant dna , lymphotoxin alpha , chemotaxis , peripheral blood , tumor necrosis factor alpha , peripheral blood mononuclear cell , monocyte , immunology , lymphotoxin beta receptor , human blood , necrosis , medicine , biology , in vitro , pathology , receptor , gene , biochemistry , physiology
Human recombinant tumour necrosis factor β (rhuTNFβ)/lymphotoxin was tested for human neutrophil granulocyte (PMN), monocytes (MO), and T‐cell chemotactic activity by means of a modified Boyden chamber system. Over a wide range of concentrations (10 ‐7 ‐10 ‐14 M) rhuTNFβ showed no chemotactic activity for PMN, MO, or T cells. In contrast, strong chemotactic migration was elicited in PMN and MO with the tripeptide N ‐formyl‐methionyl‐leucylphenylalanine (FMLP) and in T cells when complement split product C5a and leukotriene B 4 (LTB 4 ) were used as chemotaxins. The results of this study indicate that rhuTNFβ/lymphotoxin is not a chemotaxin for human PMN, MO, or T lymphocytes in vitro.