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Effect of Prolonged Monoclonal Antibody Administration on Cardiac Allograft Survival in the Rat
Author(s) -
ROZA A. M.,
KIMURA H.,
MARKMANN J.,
NAJI A.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01218.x
Subject(s) - monoclonal antibody , major histocompatibility complex , transplantation , immunology , t cell , medicine , t lymphocyte , cd8 , mixed lymphocyte reaction , andrology , antigen , biology , antibody , immune system
After heterotopic cardiac transplantation in the rat, monoclonal antibodies (MoAb) specific for rat T‐cell subsets were administered until rejection. Across combined major histocompatibility complex (MHC) and non‐MHC differences (WF to Lew) and isolated non‐MHC differences (WF to Lew.1W) cardiac allografts were rapidly rejected in unmodified hosts (7.7 ± 1.0 days and 12.2 ± 0.8 days respectively). Across combined MHC and non‐MHC differences, administration of MoAb OX‐19 (pan T‐cell) on days ‐1,0, and 1 (where day 0 was the day of transplantation) and alternate days thereafter until rejection significantly prolonged allograft survival (28.5 ± 10.2 days, P <0.01). Administration of MoAb W3/25 (helper T cell) and MoAb OX‐39 (interleukin 2 (IL‐2) receptor) prolonged allograft survival (11.3 ± 2.6 days, P <0.05 and 13.3 ± 2.0 days, P < 0.01 respectively), whereas MoAb OX‐8 (cytotoxic/suppressor T cell) administration had no effect on allograft survival. In contrast, across non‐MHC differences (WF to Lew.1W) administration of MoAb OX‐8 markedly prolonged allograft survival (85, < 100 ± 3 days) whereas MoAb W3/25 administration had no effect. The effect of MoAb administration on lymphocyte subsets at rejection was assessed by flow cytometry. The relationship between depletion of targeted T‐cell subsets and graft survival was variable. Across both combined MHC and non‐MHC and isolated non‐MHC differences MoAb OX‐8 administration resulted in a marked reduction of OX‐8 + cells at rejection with no prolongation of graft survival in the former and indefinite graft survival in the latter. In contrast, OX‐19 administration resulted in prolonged graft survival but at rejection there were significant numbers of OX‐I9 + cells present. Administration of MoAb W3/25 failed to affect a significant reduction in W3/25 + cells, but allograft survival was nonetheless prolonged.