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Detection of Individual Interleukin 4‐ and Gamma Interferon‐Producing Murine Spleen Cells after Activation with T‐Cell Mitogens
Author(s) -
SANDER B.,
CARDELL S.,
HEREMANS H.,
ANDERSSON U.,
MÖLLER G.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01216.x
Subject(s) - lymphokine , concanavalin a , interleukin 2 , microbiology and biotechnology , spleen , biology , interleukin 3 , t cell , pokeweed mitogen , interferon gamma , cytokine , immunology , il 2 receptor , immune system , biochemistry , in vitro
Murine spleen cells were activated with concanavalin A (Con A), pokeweed mitogen (PWM), or phorbol myristate acetate (PMA) and the calcium Ionophore A23187. Cells producing gamma interferon (IFN‐γ) or interleukin 4 (IL‐4) could be detected by lymphokine‐specific monoclonal antibodies and indirect immunofluorescence. The frequency and kinetics of the lymphokine‐producing cells were examined and were approximately the same after stimulation with Con A or PMA and A23187. Thirty hours after activation. 3–9% of the cells produced IFN‐γ. There were few IL‐4‐producing cells, and the maximal frequency was 1 out of 400 spleen cells 48 h after activation. When the cells were activated with PWM, the frequency of IFN‐γ‐producing cells was still high 72 h after culture. The majority of the IFN‐γ‐producing cells were CD8 + and expressed receptors for IL‐2.