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The Influence of C3‐Coated Homologous Erythrocytes on Pokeweed‐Mitogen‐Induced Polyclonal Differentiation of Human B Cells
Author(s) -
RASMUSSEN J. MØLLER,
SVEHAG S.E.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01203.x
Subject(s) - peripheral blood mononuclear cell , pokeweed mitogen , in vivo , polyclonal antibodies , in vitro , antibody , monoclonal antibody , monoclonal , microbiology and biotechnology , immunology , homologous chromosome , biology , chemistry , biochemistry , gene
The present study was undertaken in order to test the hypothesis that homologous erythrocytes (E) coated in vivo with C3d could modulate the immunoglobulin (Ig) synthesis of human peripheral blood mononuclear cells (PBMC), stimulated with pokeweed mitogen (PWM) in vitro. E from healthy individuals were found to enhance markedly the Ig synthesis of PBMC cultures stimulated with suboptimal doses (0.01 μg/ml) of PWM. E coated in vivo with increasing amounts of C3d (1.4–6.3 times the amounts on normal E), obtained from patients with systemic lupus erythematosus, failed to induce any significant increase in Ig synthesis of PBMC cultures stimulated with suboptimal PWM doses, compared with cultures co‐stimulated in parallel with normal E. In contrast, an increase in IgM and IgG synthesis was observed in about 50% of PBMC cultures from different donors when stimulated with PWM in the presence of L‐ touted with Ohm vivo (from a patient with congenital factor I deficiency), compared with the Ig synthesis in cultures co‐stimulated in parallel with normal E. In contrast to the inability of C3d‐coated E to modulate B‐cell proliferation, the monoclonal anti‐CR2 antibody OKB7 was found to be mitogenic for unstimulated peripheral B cells.