Premium
Inhibition of Human Natural Killer Cell and Lymphokine‐Activated Killer Cell Cytotoxicity and Differentiation by Vitamin D 3
Author(s) -
LEUNG K. H.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01202.x
Subject(s) - phytohaemagglutinin , lymphokine , natural killer cell , vitamin d and neurology , interleukin 12 , cytotoxicity , biology , vitamin , interferon , lymphokine activated killer cell , interleukin 2 , interferon gamma , cytokine , endocrinology , chemistry , interleukin 21 , medicine , immunology , t cell , biochemistry , lymphocyte , immune system , cytotoxic t cell , in vitro
Recent data suggest that vitamin D 3 may be capable of immunoregulation after it is converted to an active metabolite, 1,25‐dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ). The effect of vitamin D 3 and 1,25(OH) 2 D 3 on human natural killer (NK) cells and their activation by interferon (IFN) and interleukin 2(IL‐2) was investigated. Vitamin D 3 and 1,25(OH) 2 D 3 inhibited NK cytotoxicity in a dose‐dependent manner. Pretreatment of non‐adherent (NA) cells at 37°C for 18 h with the vitamins also led to inhibition of NK activity. Both the inhibition of NK lysis and pretreatment of NA cells were dependent on the concentrations of fetal calf serum (FCS) in the medium. The inhibition of NK activity was less effective in the presence of 10% FCS than with 1% FCS. Vitamin D 3 inhibited both IFN and IL‐2 activation of NK activity. However, increasing doses of IL‐2 were able in abrogate the inhibition caused by vitamin D 3 . Vitamin D 3 was able to inhibit NK activity of phytohaemagglutinin and IL‐2‐activaled cells, and also inhibit the proliferation and lymphokine‐activated killer activity induced by IL‐2. NA cells pretreated with vitamin D 3 did not respond well to IL‐2. NA cells pretreated with tow doses of IL‐2 were sensitive to inhibition by vitamin D 3 while those pretreated with high doses of IL‐2 were not. The data presented suggest that vitamin D 3 and 1,25(OH) 2 D 3 inhibit NK activity and LAK cellular differentiation.