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Differential Sensitivities of Purified Human Eosinophils and Neutrophils to Defined Chemotaxins
Author(s) -
MORITA E.,
SCHRÖDER J.M.,
CHRISTOPHERS E.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01175.x
Subject(s) - chemotaxis , leukotriene b4 , complement component 5 , platelet activating factor , chemistry , neutrophile , n formylmethionine leucyl phenylalanine , granulocyte , immunology , microbiology and biotechnology , inflammation , biology , complement system , biochemistry , in vitro , receptor , immune system
Functions of eosinophils and neutrophils isolated from normal human blood were determined by measuring chemotactic migration and release of β‐glucuronidase. Four well‐Characterized chemotaxins, the complement fragment C5a, formyl‐methionyl‐leucyl‐phenylalanine (FMLP), platelet‐activating factor (PAF), and leukotriene B 4 (LTB 4 ) were used as stimuli. Neutrophils showed remarkable chemotactic responses to all four chemotaxins. In contrast, eosinophils showed a significant chemotactic response to C5a and PAF, but only weak responses to FMLP and LTB 4 . Using these chemotaxins we found the following order of chemotactic potency (maximal number of migrated cells): C5a=LTB 4 >FMLP>PAF for neutrophils and PAF=C5a>LTB 4 =FMLP for eosinophils. Neutrophils elicited a significant β‐glucuronidase release when stimulated by C5a and FMLP, whereas only small amounts were released with PAF and LTB 4 . On the other hand, an amount of β‐glucuronidase released from eosinophils comparable to that from neutrophils was elicited only with C5a. FMLP, LTB 4 , and PAF caused the release of small percentages of β‐glucuronidase. The important cellular functions of eosinophils and neutrophils, chemotaxis and enzyme release, are thought to be controlled by differential responsiveness to stimuli.