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The Effect of Gamma Interferon on Interleukin 1 Release of Human Monocytes
Author(s) -
SIHVOLA M.,
HURME M.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01173.x
Subject(s) - lipopolysaccharide , monocyte , chemistry , interleukin , interferon gamma , isoelectric focusing , microbiology and biotechnology , interferon , size exclusion chromatography , messenger rna , biological activity , cytokine , biochemistry , biology , immunology , in vitro , enzyme , gene
The effect of gamma interferon (IFN‐γ)on interleukin 1 (IL‐1) production by human monocytes induced either by bacterial lipopolysaccharide (LPS) or silica dust was examined. Release of biologically active IL‐1 from LPS‐activated monocytes was enhanced in a dose‐dependent manner with IFN‐γ. The augmenting effect of IFN‐γ was most marked in monocytes stimulated with a sub‐optimal dose of LPS (1 ng/ml). IFN‐γ delivered the augmenting effect only if present at the beginning of the IL‐1 induction. The IL‐1 released in the presence of IFN‐γ was characterized by gel filtration on fast protein liquid chromatography (FPLC) and by isoelectric focusing; the augmented form of IL‐1 was a 17 kDa molecule, with pI 7, i.e. IL‐1β. In contrast to these data, IFN‐γ had a decreasing effect on IL‐1 release by monocytes stimulated with silica dust, in monocyte cultures stimulated with a sub‐optimal dose of silica (100 μg/ml), only minute amounts of biologically active IL‐1 were released in the presence of IFN‐γ. With higher silica concentrations the decreasing effect was less strong. The effects of IFN‐γ on the levels of IL‐1β mRNA were also analysed. IFN‐γ did not alter the accumulation of IL‐1β mRNA in LPS‐stimulated monocytes. In contrast in silica‐stimulated monocytes IFN‐γ reduced the steady‐state levels of IL‐1bT mRNA. Thus, these data indicate that, depending on the stimulating agent, IFN‐γ either down‐ or up‐regulates IL‐1 production: the former takes place mainly al the level of transcription, while the latter occurs with post‐transcriptional mechanisms.