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Induction of Interleukin 1α (IL‐1α) and IL‐1β mRNA Expression and Cellular IL‐1 Production by Anti‐HLA‐DR Antibodies in Human Monocytes
Author(s) -
PALAKAMA T.,
SIHVOLA M.,
HURME M.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01164.x
Subject(s) - monoclonal antibody , antibody , human leukocyte antigen , secretion , interleukin , microbiology and biotechnology , messenger rna , biology , interleukin 19 , monocyte , immunology , antigen , cytokine , gene , endocrinology , interleukin 5 , biochemistry
We studied the role of HLA class II antigens in the regulation of interleukin 1 (IL‐1) production in human monocytes. Monocytes were cultured with monoclonal anti‐HLA‐DR antibodies for 24 h after which cellular (i.e. intracellular and membrane‐associated) IL‐1 production, IL‐1 secretion, and the expression of IL‐1α and IL‐1β mRNA were determined. One of the anti‐HLA‐DR antibodies tested (anti‐HLA‐DR, Becton Dickinson) clearly induced IL‐1α and IL‐1β mRNA expression and cellular IL‐1 production. The other anti‐HLA‐DR antibody tested (OKIa1, Ortho) had no effect on IL‐1 production. The stimulatory effect of anti‐HLA‐DR was enhanced by IFN‐γ in both fresh and aged monocytes. A synergistic effect by anti‐HLA‐DR and suboptimal doses of LPS (1 ng/ml) on both cellular IL‐1 production and secretion was also demonstrated. The possibility of contaminating LPS causing the IL‐1‐inducing effect of anti‐HLA‐DR was excluded by the inability of polymyxin B to abolish the anti‐HLA‐DR‐induced IL‐1 production.