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Ability of Pure Resting CD8 + Human T Cells to Respond to Alloantigen
Author(s) -
LEIVESTAD T.,
HALVORSEN R.,
GAUDERNACK G.,
THORSBY E.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01157.x
Subject(s) - cytotoxic t cell , cd8 , biology , in vitro , interleukin 2 , effector , interleukin 3 , microbiology and biotechnology , stimulation , immunology , interleukin 21 , chemistry , cytokine , immune system , endocrinology , biochemistry
The ability of highly purified resting human CD8 cells to respond to alloantigens in vitro was examined. Necessary conditions for Induction of interleukin 2 receptors (IL‐2R), IL‐2 production, proliferative responses, and various effector functions were determined. Allogeneic non‐T cells induced IL‐2R expression in a high proportion of resting CD4 and CD8 cells, but only CD4 cells produced detectable amounts of IL‐2. CD8 cells also became IL‐2 responsive upon stimulation with purified resting allogeneic CD4 or CD8 cells, indicating that HLA class I + , II ‐ cells alone may initiate activation of resting CD8 cells. The activated CD8 cells needed the presence of simultaneously activated CD4 cells or exogenous IL‐2 to be able to synthesize DNA. Effector functions like cytotoxicity, mixed lymphocyte culture (MLC) suppression, or gamma interferon (IFN‐γ) production were also only detectable when the CD8 cells were activated in the presence of IL‐2.