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The Modulatory Effect of Lipoproteins on the Release of Interleukin 1 by Human Peritoneal Macrophages Stimulated with β‐1,3‐ d ‐Polyglucose Derivatives
Author(s) -
RASMUSSEN L.T.,
SELJELID R.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01147.x
Subject(s) - lipopolysaccharide , lipoprotein , chemistry , very low density lipoprotein , in vitro , endocrinology , receptor , low density lipoprotein , foam cell , cholesterol , macrophage , interleukin , medicine , pharmacology , cytokine , biochemistry , biology
Human peritoneal macrophages were stimulated in vitro with β‐1,3‐ d ‐polyglucose‐derivatized microbeads (GDM) or soluble animated 0–1,3‐D‐polyglucOK (AG) in combination with lipoproteins. The release of interleukin 1 (IL‐1) was analysed in cell supernatants in a thymocyte proliferation assay. We report that the release of IL‐1 is markedly enhanced in macrophages stimulated with polyglucose in either form in combination with native low‐density lipoprotein (LDL) or acetyl LDL at a concentration of 100 μg/ml. By increasing the amount of lipoproteins up id 10‐fold, the IL‐1 release decreased sharply. There was only a slight increase in activity when high‐density lipoprotein (HDL) or very low‐density lipoprotein (VLDL) were added. Other stimulatory agents, such as gamma interferon (IFN‐γ) and lipopolysaccharide (LPS) showed about half the activity of polyglucose. There was no significant difference between native LDL and acetyl LDL in potentiating effect. Our observations also suggest that the potentiating effect of LDL or acetyl LDL is not dependent on binding to their specific receptors. These findings provide a connection between macrophages, lipoproteins, and cytokines with regard to their role in the inflammatory response.