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Studies on the Role of CD43 in Human B‐Cell Activation and Differentiation
Author(s) -
WIKÉN M.,
BJÖRCK P.,
AXELSSON B.,
PERLMANN P.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01134.x
Subject(s) - cd43 , sialoglycoprotein , population , pokeweed mitogen , antibody , microbiology and biotechnology , biology , monoclonal antibody , b cell , immunology , lymphoblast , cell culture , medicine , in vitro , peripheral blood mononuclear cell , biochemistry , genetics , cd20 , environmental health
The monoclonal antibody (MoAb) B1B6 to human leucocyte sialoglycoprotein, CD43, induces aggregation of T cells and drivers progression signals early during activation of both T and B cells in the presence of primary activators of proton kinase C. In this report we further studied the role of CD43 in human B‐cell activation and differentiation. About 5–10% of resting tonsillar B cells are CD43 + . In the presence of TPA or antibodies to CDw40, the proportions of CD43 + cells drastically increased. The expression was optimal on day 3 of culture, when up to 80% and 50%, respectively, were CD43 + . Whereas MoAb B1B6 together with TPA induced a three‐ to fivefold higher proliferative response as compared to TPA alone, antibodies to CDw40 did not synergize with MoAb B1B6 in B‐cell proliferation Tonsillar populations depleted of CD43 + B cells responded with lower proliferation to TPA alone or to TPA and B1B6 or anti‐CDw40antibodies. MoAb BlB6 did not affect the production of IgM or IgG as induced by pokeweed mitogen in the presence of autologous T cells, from either peripheral blood or tonsillar B cells. Neither did it affect the IgG production from the CD43 + BSF‐2 sensitive Epstein Barr virus‐transformed lymphoblastoid cell line CESS. The results show that CD43 is upregulated on B cells during activation. Furthermore, CD43 + B cells are included in the population which responds to signals delivered by TPA, anti‐CD43 or anti‐CDw40 antibodies, and the proliferation of this population is not merely due to an expansion of the small population of CD43 + cells present among these cells. Moreover, the epitopes recognized by MoAb B1B6 are not involved in the differentiation of and ultimate Ig‐secretion from activated B cells.