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Trypsin Does Not Reconstitute Responsiveness to Lipopolysaccharide in the Strain C3H/HeJ, but Is a B‐Cell Mitogen‐Like Lipopolysaccharide, Stimulating a Different Subpopulation
Author(s) -
CARDELL S.,
MÖLLER G.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01110.x
Subject(s) - trypsin , lipopolysaccharide , spleen , microbiology and biotechnology , stimulation , secretion , biology , trypsin inhibitor , chemistry , immunology , endocrinology , biochemistry , enzyme
The effect of trypsin on mouse spleen cells and enriched B cells, added alone or together with lipopolysaccharide (LPS), was investigated. With trypsin, proliferation in serum free spleen cell cultures was 2–6 times greater than the background using cells from LPS responder strains, and 2–4 times the background with cells from the C3H/HeJ strain. Trypsin also induced the formation of a low number of IgM plaque forming cells (PFC). When added together with LPS, trypsin increased the proliferation caused by LPS alone by 10–50% with cells from LPS responder strains and by 50–100% with cells from the LPS non‐responder strain C3H/HeJ. Trypsin enhanced proliferation in cultures maximally stimulated by LPS. The increased proliferation obtained when trypsin was added to LPS‐stimulation of cells from the C3H/HeJ strain, was therefore not interpreted as a reconstitution of the LPS response. We conclude that trypsin has a moderate mitogenic effect on mouse B cells, stimulating the cells to proliferate and secrete IgM. The mechanism of action is unknown, but is different and independent from the action of LPS.