Premium
Recombinant Tumour Necrosis Factor (TNF) Fixed to Cell Monolayers Retains Its Cytotoxic and Growth‐Stimulatory Activity
Author(s) -
HOFSLI E.,
NISSENMEYER J.
Publication year - 1989
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1989.tb01099.x
Subject(s) - cytotoxic t cell , internalization , tumor necrosis factor alpha , cell culture , cytotoxicity , biology , clone (java method) , recombinant dna , microbiology and biotechnology , cell , second messenger system , paraformaldehyde , receptor , endocrinology , medicine , signal transduction , in vitro , biochemistry , chemistry , genetics , gene , organic chemistry
Recombinant human tumour necrosis factor (rTNF) retained its cytotoxic activity after being fixed with paraformaldehyde to adherent cell monolayers. The cytotoxicity appeared to be mainly due to fixed rTNF and not to any free soluble rTNF that could have leaked out from the lived rTNF cell preparations. The fixed rTNF cell preparations also stimulated the growth of human diploid fibroblasts, under conditions where little growth‐Stimulatory activity was found in suspension. These results indicate that TNF may exert its effect on target cells without internalization, perhaps through a receptor‐mediated process that may alter the levels of a second messenger within the target cells. This signal transduction does not appear to involve cAMP or cGMP, since we were unable to detect significant changes in the levels of these two second messengers in TNF‐exposed WEHI 164 clone 13 cells.