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Monoclonal Antibodies against the Major Internal Protein, p27, of a Psoriasis‐Associated Retrovirus‐Like Particle
Author(s) -
ÅSBAKK K.,
IVERSEN O.J.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02431.x
Subject(s) - monoclonal antibody , retrovirus , psoriasis , virology , antibody , medicine , immunology , virus
Four murine monoclonal antibodies (MoAb) were raised against p27 isolated from psoriatic scale. The MoAb recognized the antigen on a subfraction (0.1–1%)of psoriatic lymphocytes as well as in skin biopsies from psoriatic patients. In contrast, the antigen could not be detected on lymphocytes or in skin biopsies from healthy controls. A sandwich ELISA assay using the MoAb for detection of p27 is described. This method, together with a competition antibody‐binding assay for distinction of epitopes, demonstrates that the MoAb recognize lour different antigenic determinants on p27.