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Cytomegalovirus‐Infected Adherent Cells Interact Synergistically and Antagonistically with Staphylococcus aureus Protein A in vitro
Author(s) -
PAULIN T.,
RINGDÉN O.,
WAHREN R.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02429.x
Subject(s) - staphylococcus aureus , in vitro , microbiology and biotechnology , cytomegalovirus , biology , virology , chemistry , bacteria , virus , biochemistry , herpesviridae , viral disease , genetics
Cytomegalovirus (CMV) has been shown to exert suppressive effects on the immune response but also to have mitogenic properties. A bacterial product, protein A from Staphylococcus aureus (SpA) was chosen to study possible interaction in vitro between bacterial products and adherent cells incubated with infectious CMV and ultraviolet light (UV)‐inactivated CMV. Small amounts of infectious CMV potentiated SpA‐induced DNA synthesis and Ig secretion measured by induction of plaque‐forming cells (PFC). The reason for this may be that CMV in small amounts may act in synergism with the non‐specific mitogen SpA. UV‐inactivated CMV did not influence these responses except for a markedly enhanced PFC induction with SpA in lymphocytes from seronegative individuals. This remarkable synergism with SpA was also seen in enriched B cells. No synergism was seen in lymphocytes from seropositive donors. Large amounts of infectious CMV markedly reduced SpA‐induced immune responses. Preliminary data suggest that the immunosuppressive effects are mediated by an interleukin l inhibitory factor. CMV was not shown to be a polyclonal B‐cell activator but may, possibly in small amounts, act as such together with bacterial products, which would explain certain autoimmune phenomena. To conclude, CMV could in interaction with a bacterial product generate both synergistic and suppressive effects on immune response.