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Role of CD8 (Lyt‐2) in Cytotoxic T‐Cell Function
Author(s) -
MUNAKATA T.,
SCHMIDTULLRICH R.,
BERTSCHMANN M.,
EICHMANN K.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02417.x
Subject(s) - cytotoxic t cell , ctl* , cytolysis , antigen , cd8 , clone (java method) , cytotoxicity , biology , effector , t cell , immunology , function (biology) , microbiology and biotechnology , immune system , in vitro , biochemistry , gene
The role of CD8 (Lyt‐2) in the function of a long‐term cytotoxic T‐cell (CTL) clone (C196) was analysed. Previous studies had shown that C196 cells utilize the αβT‐cell receptor and depend on Lyt‐2 to recognize and lyse P815 mastocytoma target cells. Recognition is H‐2K d restricted, presumably involving a P815 specific antigenic structure. Here we analyse a number of variants selected from C196 that have reduced or virtually abolished expression of Lyt‐2, alone or in combination with other deficiencies. We studied the ability of these variant cells to lyse either P185, a process requiring both specific antigen recognition and triggering of cytolytic function, or to lyse the anti‐CD3 hybridoma 145‐2C11, a process that requires the triggering of cytolytic function only. The results shows that in the case or a permanently activated CTL, effector cell such as C196, Lyt‐2 is required for antigen recognition but is not essential for the triggering of cytotoxicity.