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Leukaemic B Cells from Patients with Chronic Lymphocytic Leukaemia Suppress Immunoglobulin Production by Lymphocytes from Normal Donors
Author(s) -
KUNICKA J.,
PLATSOUCAS C. D.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02409.x
Subject(s) - antibody , pokeweed mitogen , immunology , monoclonal antibody , chronic lymphocytic leukemia , immunoglobulin m , b cell , surface immunoglobulin , biology , immunoglobulin g , microbiology and biotechnology , leukemia , peripheral blood mononuclear cell , in vitro , biochemistry
We observed that highly purified E‐rosette‐negative largely leukaemic B cells from 9 out of 15 patients with chronic lymphocytic leukaemia (CLL) significantly suppressed immunoglobulin production by mixtures of T4 and B cells from normal donors in the presence of pokeweed mitogen (PWM). This suppression by leukaemic B cells was concentration‐dependent. Addition of equal numbers of B cells from normal donor to the mixtures of normal T4 and B cells increased, or had no effect on the production of IgM, IgA, and IgG Treatment of purified largely leukaemic B cells from patterns with CLL with either the anti‐B1 or anti‐Leu 1 monoclonal antibody plus complement abolished their ability to suppress immunoglobulin production. In contrast, treatment with either the anti‐Leu 5 or the OKMI monoclonal antibody plus complement had no effect on the suppression. These results tugged that leukaemic B cells from certain patients with CLL may exhibit, or can be induced to exhibit, immunosuppressive properties.