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T Cell‐Mediated Production of Tumour Necrosis Factor‐α by Monocytes
Author(s) -
DEBETS J. M. H.,
LINDEN C. J.,
SPRONKEN I. E. M.,
BUURMAN W. A.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02388.x
Subject(s) - necrosis , tumor necrosis factor alpha , monocyte , immunology , cell , biology , cancer research , medicine , pathology , genetics
The aim of this study was to examine the tumour necrosis Factor (TNF)‐α production by peripheral blood mononuclear cells activated by mitogens. Considerable amounts of TNF‐α, ranging from 1.0 to 5.0 ng/ml, were present in the supernatants of cultures of human peripheral blood mononuclear cells (PBMC), stimulated with either the anti‐CD3 monoclonal antibodt OKT3 or the lectin phytohaemagglutinin (PHA). The amount of TNF‐α secreted in the supernatant was closely correlated to the degree of T cell proliferation in such cultures, as measured by [ 3 H]TdR incorporation. In the absence of proliferating T cells the mitogens did not induce secretion of TNF‐α by monocytes. Supernatant of proliferating T cells were shown to induce TNF‐α production by monocytes. The macrophage‐activating factor gamma interferon (IFN‐γ) was also shown to induce, in the absence of endotoxin, TNF‐α secretion by monocytes in a dose‐dependent manner. The induction of TNF‐α production by supernatants of proliferating T cells could largely be abrogated by passaging the supernatants on an anti‐IFN‐γ column before adding them to the monocytes. It is therefore concluded from this study that the production of TNF‐α by monocytes can be induced by proliferating T cells and that this induction can largely be attributed to the T cell lymphokine IFN‐γ.