Premium
Inhibitor of Interleukin 1 in Normal Human Urine
Author(s) -
SVENSON M.,
BENDTZEN K.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02387.x
Subject(s) - urine , immunology , interleukin , medicine , cytokine
We investigated whether urine from normal individuals contains inhibitors of interleukin 1 (IL‐1). Diafiltered urine from normal afebrile donors suppressed IL‐1‐induced interleukin 2 (IL‐2) activity of mouse thymocyte supernatants. These supernatants, however, strongly suppressed IL‐2‐induced [ 3 H] thymidine incorporation into the IL‐2‐sensitive cell line CTLL.‐2, whereas the urine preparations did not. This phenomenon was caused by an increased amount of thymidine secreted by the urine‐treated thymocytes. Therefore, in order to prevent interference, experiments were carried out with excess [ 3 H]thymidine. Under these circumstances, suppression of IL‐1‐ and to a lesser extent IL‐2‐induced DNA synthesis was still observed, whereas the synergistic effect of IL‐1 on IL‐2‐induced DNA synthesis was only marginally reduced. We conclude that suppression of IL‐1‐induced IL‐2 production by mouse thymocytes is a major effect of the IL‐1‐inhibitory factor(s)in normal urine. When the murine EL 4 cell line was used, the diafiltered urine failed to inhibit IL‐1‐induced IL‐2 production. The detection of an IL‐1 inhibitor in urine is therefore dependent on the target cells as well as the effects of IL‐1 on these cells.