Premium
Repopulation Potential of Thymocytes Forming Rosettes with Phagocytic Cells of the Thymic Reticulum
Author(s) -
EL ROUBY S.,
EZINE S.,
PAPIERNIK M.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02368.x
Subject(s) - homing (biology) , spleen , thymocyte , microbiology and biotechnology , biology , monoclonal antibody , immunofluorescence , in vitro , congenic , reticulum , immunology , lymph node , endoplasmic reticulum , chemistry , antibody , t cell , immune system , biochemistry , ecology , gene
Thymocytes binding in vitro to phagocytic cells of the thymic reticulum (P‐TR), termed ‘rosetting thymocytes’, were injected intravenously into irradiated congenic mice and their migration patterns were compared with those that do not bind to P‐TR, called ‘non‐rosetting thymocytes’, similarly transferred. Donor cells, C57BL/Ka Thy 1.2, were distinguished from recipient cells, C57BL/Ka Thy 1.1 by a direct immunofluorescence technique using an anti‐Thy 1.2 monoclonal antibody. The results demonstrate that the rosetting thymocytes have a greater capacity for homing back to the thymus and for populating the mesenteric lymph node and the spleen. Intrathymic transfer assay revealed that the donor‐derived cells detected in the peripheral organs were of thymic origin.