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Correlation of Susceptibility and Cytostatic Factor‐Inducing Activity of Tumour Cells to Peritoneal Macrophages
Author(s) -
HASHIMOTO S.,
NAGAOKA M.,
YOKOKURA T.,
MUTAI M.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02346.x
Subject(s) - concanavalin a , mannose , microbiology and biotechnology , lactobacillus casei , trypsin , biology , glycoprotein , lectin , chemistry , biochemistry , in vitro , enzyme , fermentation
L929, 3T12‐3, B16, 3LL, and YAC1 cells with cytostatic factor (CF)‐inducing activity from Lactobacillus casei ‐elicited murine peritoneal macrophages (LCEPM) were susceptible to the cytostatic activity of LCEPM and to LCEPM‐produced CF, but L1210, P388D1, and Colon 26 cells, which have no CF‐inducing activity, were resistant to that of LCEPM and to the CF. The resistance of P815 cells to that of LCEPM was stronger than that of 3T12‐3 cells, but the CF‐inducing activity of P815 cells was about 5098 weaker than that of 3T12‐3 cells. Release of CF from LCEPM was also caused by heat‐killed (100°C, 10 min) 3T12‐3 or P813 cells, and this release was inhibited by d ‐mannose. The CF‐inducing activity of heat‐killed 3T12‐3 or PK15 cells was reduced by mild trypsin digestion (37°C for 10 min). A d ‐mannose‐containing glycopeptide or glycoprotein (GP) was separated from 3T12‐3 or P815 cells by concanavalin A (Con A) or wheat germ agglutinini (WGA) affinity chromatography. The CF were released from LCEPM by stimulation with the Con A‐binding GP of the tumour cells, but the WGA‐binding GP had little activity. It is suggested that tumour cells with CF‐inducing activity may he susceptible to the cytostatic activity of LCEPM, and those without CF‐inducing activity may be resistant to the cytostatic activity of LCEPM and the release of CF from activated macrophages may be caused by the Con A‐binding GP of the tumour cell surface.