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Effects of Culture Substrates and Normal Hepatic Sinusoidal Cells on in Vitro Hepatocyte Synthesis of Apo‐SAA
Author(s) -
SUBRAHMANYAN L.,
KISILEVSKY R.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02345.x
Subject(s) - hepatocyte , in vitro , microbiology and biotechnology , chemistry , biology , medicine , biochemistry
Primary hepatocyte cultures synthesize apo‐SAA upon stimulation with supernatant from lipopolysaccharide (LPS)‐treated macrophages. The matrices on which the hepatocytes were grown influence their basal apo‐SAA synthetic capability. Fibronectin was superior. Coculturing hepatocytes with hepatic sinusoidal cells did not adversely affect the ability of hepatocytes to synthesize and secrete apo‐SAA into the culture medium. In 72 h, clear islands of endothelial cells nestled in layers of hepatocytes. Both apo‐SAA, and apo‐SAA, were made in considerable quantities but no evidence could be obtained that the apo‐SAA were free of apo‐A‐1. The coculturing of hepatocytes with liver sinusoidal cells, the site of ultimate AA deposition, is a first step in establishing an in vitro system for AA amyloidogenesis.