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Early Changes in Quiescent B Cell Physiology Subsequent to Cognate and Bystander Interaction with Helper T Cells
Author(s) -
CAMBIER J. C.,
JULIUS M. H.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb02323.x
Subject(s) - bystander effect , cognate , biology , microbiology and biotechnology , cell , immunology , physiology , genetics , linguistics , philosophy
We have assessed early changes in quiescent B cells Following cognate and bystander interaction with cloned helper T cells. Variables monitored include Ia expression, blastogenesis, G 0 to G 1 transition, and progression through cycle. We have also assessed the antigen specificity. In restriction, and dependence on membrane immunoglobulin cross‐linking of both generation and delivery of effectors that mediate B cell response. The results demonstrate that antigen presentation by quiescent B cells to T cells resulting in the generation of effectors that activate B cells is Ia‐restricted and dependent on antigen and an (mIgM and mIgD) crosslinking signal However, once generated, T cell effector functions act independently of Ia haplotype to promote Ia expression, blastogenesis, and G 0 to G 1 , transition by most small B cells. Although these responses can be mediated in T cell supernatants, further progression of B cells through S, G 2 , and M is only efficient when T h cells are present in cultures. Thus, results suggest that one or more Ia unrestricted, labile and/or cell‐associated factors, not active in most conventional T cell supernatants, are necessary to stimulate proliferation of small B cells.