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Kupffer Cells May Autoregulate Interleukin 1 Production by Producing Interleukin 1 Inhibitor and Prostaglandin E 2
Author(s) -
SHIRAHAMA M.,
ISHIBASHI H.,
TSUCHIYA Y.,
KUROKAWA S.,
HAYASHIDA K.,
OKUMURA Y.,
NIHO Y.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb01505.x
Subject(s) - prostaglandin e , thymocyte , prostaglandin e2 , endocrinology , interleukin , medicine , prostaglandin , lipopolysaccharide , kupffer cell , biology , chemistry , cytokine , immunology , t cell , immune system
Rat Kupffer cells stimulated with bacterial lipopolysaccharide (LPS) produced high levels of interleukin 1 (IL‐1), as determined by thymocyte proliferation assay. Indomethacin revealed a dose‐dependent augmentation in IL‐l production, in parallel with a dose‐dependent reduction in prostaglandin E 2 production by Kupffer cells. The addition of exogenous prostaglandin E 2 , dibutyryl cAMP, or isoproterenol led to a dose‐dependent suppression of IL‐I production. The supernatant from UPS‐stimulated Kupffer cells also contained factors that Inhibited IL‐1‐induced thymocyte proliferation. Upon gel filtration, two inhibitory peaks, at apparent MW of 27,000 and 6000, were obtained. The latter but not the former fraction also affected Interleukin 2 (IL‐2)‐induced thymocyte proliferation. Increasing amounts of IL‐1 overcame the inhibitory activity derived from the 27,000 MW fraction. These results suggest to us that prostaglandin E 2 and IL‐1 inhibitor released by Kupffer cells may be involved in negative self‐control in regulating IL‐1 production and its action.