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Effective Inhibition of Cardiolipin‐Binding Antibodies in Gram‐Negative Infections by Bacterial Lipopolysaccharide
Author(s) -
VAARALA O.,
VAARA M.,
PALOSUO T.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb01493.x
Subject(s) - cardiolipin , lipid a , lipopolysaccharide , antibody , antigen , microbiology and biotechnology , gram negative bacteria , escherichia coli , immunology , immunoassay , immunoglobulin m , biology , immunoglobulin g , biochemistry , phospholipid , membrane , gene
Anticardiolipin antibodies (ACA) were detected by solid‐phase enzyme immunoassay in the majority of sera from patients with Gram‐positive and Gram‐negative bacterial infections. The response involved all the major immunoglobulin classes IgG, IgM, and IgA. The specificity of the ACA was studied in competitive inhibition experiments with three putative antigens: cardiolipin, lipopolysaccharide (LPS) isolated from Salmonella minnesota , strain Re 595, and synthetic Escherichia coli lipid A. The binding of IgG class ACA from the sera of five patients with Gram‐negative infections was effectively inhibited by LPS, whereas 100‐fold more cardiolipin was required for comparable inhibition. Pure lipid A was a less effective inhibitor of anti‐cardiolipia activity than LPS. This pattern of reactivity was not seen in sera from patients with Gram‐positive infections, syphilis, or systemic lupus erythematosus. Our findings suggests that cardiolipin may not be the inducing antigen for the cardiolipin‐binding antibodies that develop in Gram‐negative infections.