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Most B Cells in Acute Trypanosoma cruzi Infection Lack Parasite Specificity
Author(s) -
MINOPRIO P.,
BURLEN O.,
PEREIRA P.,
GUILBERT B.,
ANDRADE L.,
HONTEBEYRIEJOSKOWICZ M.,
COUTINHO A.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb01487.x
Subject(s) - trypanosoma cruzi , parasite hosting , biology , isotype , antibody , antigen , western blot , virology , immunofluorescence , immune system , immunology , microbiology and biotechnology , monoclonal antibody , gene , biochemistry , world wide web , computer science
The specificity of B lymphocytes activated in the acute phase of murine Trypanosoma cruzi infection was analysed in a panel of immunoglobulin‐secreting hybridomas derived by fusion of lymph node cells 7 days after intraperitoneal parasite inoculation. The immunoglobulin isotype distribution of the hybrids reflected the total plaque‐forming cell (PFC) response in the animal at this point, with a predominance of IgG2a, IgM, and IgG2b. Screening of the hybridoma antibodies on parasite antigens by three independent methods (western blot, ELISA, and immunofluorescence) revealed only one (out of a total of 51) that bound a parasite molecule with an apparent molecular mass of 180 kDa. In contrast, antibodies of both IgM and IgG classes were found to react with a panel of autologous antigens. These results establish that most B cells activated by T. cruzi infection are not specific for parasite antigens and therefore indicate the relevance of analysing the totality of host responses to infection.