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Oestrogen and Human T Lymphocytes: Presence of Specific Receptors in the T‐Suppressor/Cytotoxic Subset
Author(s) -
STIMSON W. H.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb01459.x
Subject(s) - cytotoxic t cell , cd8 , monoclonal antibody , receptor , biology , suppressor , microbiology and biotechnology , t lymphocyte , cytosol , antibody , endocrinology , medicine , in vitro , antigen , immunology , biochemistry , enzyme , gene
Human spleen cells were investigated for specific oestrogen binding using oestradiol‐17β. High affinity, low capacity binding was observed ( K d =2.5±10 −10 m ) and the characteristics of the oestrogen receptor identified were typical of this type of species. T lymphocyte subpopulations were prepared by negative selection by means of the monoclonal antibodies Leu 2a and 3a. Oestrogen receptors were only found in T cells of the suppressor/cytotoxic (CD8‐positive) subset while helper/inducer lymphocytes (CD4‐positive) showed no significant steroid binding by cytosol and nuclear assays. These results are supported by data from experiments into the effects of oestradiol‐17β on immunoglobulin secretion, where it was apparent that only the suppressor/cytotoxic T‐cell subpopulation was responsive to the presence of the steroid.