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Identification of a Human C5 β‐Chain Epitope Exposed in the Native Complement Component but Concealed in the SC5b‐9 Complex
Author(s) -
MOLLNES T. E.,
KLOS A.,
TSCHOPP J.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb01453.x
Subject(s) - epitope , monoclonal antibody , antibody , immunization , clone (java method) , chemistry , complement (music) , blot , microbiology and biotechnology , zymosan , biochemistry , biology , immunology , in vitro , phenotype , dna , complementation , gene
Monoclonal antibodies obtained after immunization of mice with human C5 were screened for activity against native C5 and SC5b‐9. Clone 568 reacted with the C5 β‐chain in western blotting and with purified native C5 in double antibody enzyme immunoassays. The reactivity of done 568 against serum decreased markedly when serum was activated with zymosan. The residual activity was found to be against free C5, whereas the antibody did not read at all wish the SC5b‐9 complex. Thus, the antibody 568 reacts with an activation‐dependent C5 epitope, which is concealed when C5 is incorporated into the terminal complement complex