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T‐ and B‐Cell Functions in IgA‐Deficient Patients
Author(s) -
KLEMOLA T. K.,
ESKOLA J.,
SAVILAHTI E.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb01452.x
Subject(s) - pokeweed mitogen , phytohaemagglutinin , peripheral blood mononuclear cell , concanavalin a , lymphocyte , immunology , in vitro , antigen , b cell , endocrinology , cd8 , biology , microbiology and biotechnology , medicine , antibody , chemistry , biochemistry
In vitro lymphocyte function of 60 IgA‐deficient patients(IgAdp) were studied. In the mitogen‐induced lymphocyte activation test, peripheral blood mononuclear cells (PBMC) of IgAdp showed a weaker response to pokeweed mitogen (PWM) than those ill controls ( P <0.05), and the responses to phytohaemagglutinin (PHA) and concanavalin A (Con A) were normal. The amounts of IgA, IgG, and IgM secreted by PBMC from controls and IgAdp were measured in vitro by enzyme immunoassay. B cells were activated by PWM with or without hydrocortisone (HC) to inhibit HC‐sensitive suppressor cells. Lymphocytes of IgA‐deficient patients synthesized only minute amounts of IgA in vitro and the amounts of IgA synthesized correlated well with the serum IgA levels. Both IgG and IgM secretion by PWM‐Stimulated PBMC of IgAdp were also subnormal. The lymphocyte subset analysis in the peripheral blood revealed normal numbers and ratios of T cells, but IgAdp had reduced percentages of surface IgA‐bearing cells. Co‐culture experiments with isolated B cells. CD4 + and CD8 + cells from IgAdp, and controls showed low B‐call capacity for IgA production as the most constant finding Defects in T‐cell functions or chances in the proportions of surface antigen‐carrying lymphocytes (other than IgA + ) were infrequent and were not associated with any particular group of patients or type of IgAd (primary, acquired, or transient).