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Thymic Accessory Cells Required for the Increased Responsiveness of Thymocyte Subpopulations to Interleukin 1‐Like Monokine in Guinea Pigs
Author(s) -
AIDA Y.,
KAKIMOTO K.,
KAWAKAMI K.,
CHIBA J.,
AONO M.,
ONOUE K.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb01450.x
Subject(s) - monokine , thymocyte , biology , endocrinology , medicine , stimulation , guinea pig , t lymphocyte , immunology , t cell , interleukin , immune system , cytokine
The proliferative response of guinea‐pig thymocytes to co‐mitogenic stimulation with phytohaemuglutinin and the guinea‐pig interleukin 1 (IL‐1)‐like lymphocyte‐activating monokine was lost by removing the cells that adhere to a Sephadex G‐10 (G‐10) column or the cells of low density in a Ficoll‐Conray gradient. The diminished response in the G‐10 non‐adherent thymocyte or high‐density thymocyte fraction was restored by the addition of a macrophage‐depleted 6–10 adherent thymocyte fraction or a low‐density. Ia‐positive thymocyte fraction but not by the addition of peritoneal macrophages. These results suggest that the accessory cells which mediate the increased responsiveness of thymocytes to the IL‐1‐like monokine existed in G‐10 adherent cell fractions and the cells with this accessory function were not macrophages The accessory cells were shown to DC of low density, glass‐non‐adherent, G‐10‐adherent, Fc receptor‐negative, and Ia‐positive. These results also suggest that the G‐10‐non‐tidherent and high‐density thymocyte subpopulation, which is unresponsive or responds very little to the IL‐l‐like monokine by itself, acquires responsiveness to the monokine and proliferates by Stimulation with the 1L‐1‐Iike monokine and lectin in the presence of the accessory cells.