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Accessory Cell‐Dependent T‐Cell Activation via Ti‐CD3
Author(s) -
HALVORSEN R.,
LEIVESTAD T.,
GAUDHRNACK G.,
THORSBY E.
Publication year - 1988
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1988.tb01449.x
Subject(s) - cd3 , cd8 , t cell , microbiology and biotechnology , monoclonal antibody , biology , interleukin 2 , cell culture , t cell receptor , receptor , antigen , immune system , antibody , immunology , biochemistry , genetics
The activation of resting T cells to interleukin 2 (IL‐21) production and DNA synthesis via T1‐CD3 is dependent on accessory cells (AC) using positively selected, resting I cells activated with particle‐bound anti‐CD3, we investigated the ability of various cell lines to function as AC. We found that cell lines able to act as AC all expressed LFA‐3, while cell lines not expressing LFA‐3 were unable to provide AC signals. This applied to CD3 + . CD4 + , and CD8 + T cells. Sheep red blood cells (SRBC), which express LFA‐3‐like molecules, also had a weak, hut significant AC function in this test system. Both CD4 + and CDS + T cells activated with particle‐bound anti‐CD3 could be induced to enter DNA synthesis in the absence of AC when monoclonal antibodies reacting with CD2 were present instead of AC IL‐2 production could be detected in the latter cultures but not when positively selected CD3 + or CD2 + T cells were cultured alone. Our data suggest that activation of resting T cells via CD3 will lead to IL‐2 receptor expression, while the interactions between LFA‐3 and its ligand CD2 provide the necessary secondary signals for IL‐2 production and induction of DNA synthesis