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Differences in the Kinetics of Histamine Formation and Granulation of Human Basophilic Cells from Bone Marrow, Peripheral Blood, and Cord Blood
Author(s) -
AHLSTEDT S.,
HAMMARSTROM I.,
INTOMALMBERG M.B.,
LEHTONEN P.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02298.x
Subject(s) - histamine , basophilic , bone marrow , cord blood , endocrinology , cell culture , andrology , biology , medicine , immunology , chemistry , microbiology and biotechnology , pathology , genetics
Human bone marrow, cord blood, and peripheral blood comntain progenitor cells, which during culture mature to histamine‐containing basophilie cells. In bone marrow the histamine content per Alcian blue staining basophilie cell was low before culture. Cultivaiion of BML cells resulted in increased histamine levels in cultures(P<0.05). whereas the basophilic cells did not increase significantly. Cell cultures were stimulated with conditioned medium (CM) produced with allergen‐slimulated cells from atopies and from the Mo T leukaemie cell line. Cells from cultures slimulaled with CM contained less histamine calculated per basophilie cell than did those from unstimulted cultures (P<5.05), There was a significant correlation between the numbers of basophilic cells and the histamine content in cells on day 0 prior to cultivation and after 14 days of cultivation (P<0.01 and P<0.05 respectively). In cord blood there was a correlation between the numhers of basophilie cells and the histamine levels prior to cultivation (P<0.05). During cultivation the number of basophilie cells increased five‐lold (P<0.02), whereas the histamine levels did not inercase resulting in a decreased histamine level per basophilie cell (P<0.02). In peripheral blood the basophilic cells contained the highest levels of histamine. The numbers of hasophilie cells and their conntent of hisiamine showed good correlation both hefore and after unstimtilated and stimulated cultivation (P<0.01). whereas unstimulated cultures did not show such correlation. The results indicate the presence of different proportions of progenitor cells in bone marrow, cord blood. and peripheral blood. all with different ability to produce histamine and become granulated basophilic cells.