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Fibroblast Growth‐Stimulatory Activity Released from Human Monocytes
Author(s) -
AUSTGULEN R.,
ESPEVIK T.,
NISSENMEYER J.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02297.x
Subject(s) - fibroblast , microbiology and biotechnology , fibroblast growth factor , biology , chemistry , immunology , cell culture , genetics , receptor
Human monoeytcs release fibroblast growth‐stimulatory activity. In this study tumour necrosis factor (TNF) has been identified as a major contributor to the monocyte‐derived fibroblast growth‐stimulaiory activity. A neutralizing monoclonal antibody (MoAb) against recombinant TNF (rTNF) inhibited growth of FS‐4 fibroblasts and skin biopsy fibroblasts induced by monocyte supernatants, indicating that TNF was involved in the stimulation. Optimal growth of FS‐4 fibroblasts was induced by monocyte supernatants at dilutions which cointained TNF at a concentration between 1.6 × 10 −6 and 4.0 × 10 −5 μg/ml, less growth being induced at higher TNF concentrations. Contrary to this, no optimal concentration interval was found lor rTNF, since increasing rTNF concentrations always resulted in increased growth stimulation. It also appeared that natural TNF in the monocyte supernatants induced growth at a much lower concentration than rTNF tested in the absence of monocyte supernatant.