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Interstitial Pneumonitis and Hepatitis after Transfer of Bone Marrow Cells Bearing the Ipr Gene to Irradiated Recipients: A Disease Due to Large Granular Leueocytes?
Author(s) -
PIGUET P. F.,
IZUI S.,
JANINMERCIER A.,
KAPANCI Y.,
VASSALLI P.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02295.x
Subject(s) - bone marrow , medicine , pneumonitis , hypersensitivity pneumonitis , hepatitis , immune system , antibody , immunology , cirrhosis , pathology , lung
Mice bearing the ‘auto‐immune’ Ipr gene develop a lympho‐proliferative disease associated with the production of various antibodies, Lethally irradiated recipients were grafted with bone marrow cells (BMC) from syngeneie mice with or without the Ipr gene. After 6 months, the survivors were 0/24 and 16/20 for the recipients of lpr and normal BMC respectively. The mortality rate was independent of the presence of T lymphocytes among the BMC. Histological evaluation showed that hepatitis, interstitial pneumonitis. and sclerosis of lymphohaemopoietic organs were the major causes of death for the recipients of lpr BMC, Hepatitis was associated with an increase in the number of liver interstitial cells (LIC) from about 2x10 6 ) up to about 10 7 cells per liver. The LIC associated with the hepatitis were composed of polymorphonuclear leucocytes and large mononuclear leueocytes, showing phenotypic (i.e. Thy. 1 − , asialo GM1, presence of cytoplasmic granules) and functional (i.e, non‐phagocytic and cylolytic) properties of NK cells. The disease can be distinguished both from the spontaneous disease of the lpr mice (by the absence of‘ lpr cells'and of anti‐DNA antibodies) and from craft versus host disease hy the absence of cutaneous and intestinal lesions. It may represent a model of tissue injury mediated by large granular leucocytes.