Premium
Two M315 Idiotopes Defined by Isologous Monoclonal Antibodies: One Depends on Germline and the Other on Mutated Murine λ Light Chain Sequences
Author(s) -
KRISTOFFERSEN G.,
HANNESTAD K.,
HANSEN T.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02287.x
Subject(s) - idiotopes , germline , immunoglobulin light chain , monoclonal antibody , biology , antibody , monoclonal , genetics , microbiology and biotechnology , gene
We have previously reported that T helper cells of BALB/c mice recognize the unique mutated sequence Phe 91 , Arg 95 , Asn 96 of the λ2 L chain of isologous (BALB/c) myeloma protein 315. Here we study two Id(Id‐315.1.4 and Id‐315.TH) of the DNP‐Lys binding M315 defined by two monoclonal isologous anti‐Id Ab (Ab 2‐1.4 and Ab 2‐TH). Both Id were (1) totally expressed by Fv‐315, but not by free unpaired V domains, (2) specifically dependent on V H , since λ2‐315 recombined with four other H chains did not express the Id, (3) related to the haptenbinding site because their expression was blocked by the haptens DNP‐Lys and DNP‐Gly, and (4) topographically related because Ab 2‐1.4 and Ab 2‐TH competed with each other for binding to M315. The contribution of λ chain V regions was studied with the aid of reconstituted Ig molecules of H‐315 paired with λ1, λ2, and #LD3 L chains. Id‐315. TH was expressed equally well by reconstituted Ig containing three different λ2 chains (λ2‐5‐7, λ‐T952, and λ2‐315), but its expression was profoundly reduced when H‐315 was associated with #LD‐SAPC15 or #LD2‐T952 differ from λ2‐315 at positions 38, 94, 95, 96, and 98 or 99, respectively, Id‐315.1.4 probably requires the unique mutated amino acids Phe 94 , Arg 95 , Asn 96 of λ2‐315. This resembles the effects on Id expression of previously reported unique amino acids of the D region. We failed to confirm that hyperimmunization of BALB/c mice with Ab 2‐1.4 cross‐linked to KLH induced M315‐like Ab. The results are discussed in terms of the contribution of the third bypervariable loop of λ chains to Id and the immunogenicity of isologous Ig.