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Copper(II)(3,5‐Diisopropylsalicylate) 2 Accelerates Recovery of B and T Cell Reactivity Following Irradiation
Author(s) -
SODERBERG L. S. F.,
BARNETT J. B.,
BAKER M. L.,
SALARI H.,
SORENSON R. J.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02283.x
Subject(s) - spleen , concanavalin a , lipopolysaccharide , stimulation , immunocompetence , irradiation , immune system , andrology , immunology , t cell , microbiology and biotechnology , chemistry , endocrinology , medicine , biology , biochemistry , in vitro , physics , nuclear physics
Copper(II)(3,5‐diisopropylsalicylate) 2 (Cu‐DIPS), administered subcutaneously to mice at 80 mg/kg body weight, bad marked radioprotective activity. Given 3 h before exposure to 8.0 Gy (800 rad) irradiation. Cu‐DIPS increased the 42‐day survival from 40% to 86%. Seven days after exposure to 8.0 Gy, there were severe reductions in spleen weight (73%) and cellularity (98%) in both Cu‐DIPS‐and vehicle‐treated mice. Viable spleen cells collected 7 days after irradiation were totally unresponsive to mitogenic or antigenic stimulation regardless of Cu‐DIPS or vehicle treatment, suggesting that Cu‐DlPS did not prevent radiation‐induced damage to mature lymphocytes. At 14 days, when Cu‐DIPS‐treated mice started to show improved survival over vehicle‐treated mice, spleen weights and cellularity were 2.5‐ and 3.5‐fold higher, respectively, in Cu‐DIPS‐treated mice. Treatment with Cu‐DIPS not only enhanced splenic repopulation. but also accelerated the reappearance of both B and T cell reactivities. Spleen cell responsiveness to the B cell mitogen, lipopolysaccharide (LPS), and the T cell mttogen, concanavalin A (Con A), regenerated significantly faster in Cu‐DIPS‐treated mice. Cu‐DIPS also significantly accelerated the regeneration of T‐dependent antibody induction. Based on these assays of immunocompetence, Cu‐DIPS‐treated mice had, on average, a seven‐fold greater capacity to respond to immune stimulation than vehicle‐treated mice 24 days after irradiation.