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Functional Characterization of Human B Cells Carrying the Lymphocyte Large Sialoglycoprotein gpl50
Author(s) -
WIKÉN M.,
ROBERTSSON E.S.,
AXELSSON B.,
PERLMANN P.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02281.x
Subject(s) - biology , microbiology and biotechnology , population , lymphocyte , immunology , sociology , demography
Human B cell‐enriched populations were prepared from buffy coats of healthy donors. By means of affinity chromatography, the B cells were separated into two fractions, one enriched in and the other depleted of cells expressing gpl50, the large sialoglycoprotein of lymphocytes. In the presence of autologous T cells, monocytes and pokeweed mitogen B cell populations enriched for gpl50 + cells gave rise to significantty more plasma cells (clg + cells) and secreted significantly more IgG than gpl50‐depleted populations. In contrast, more or an equal amount of IgM was secreted in cultures containing gpl50‐depleted cells. The differences between the fractions could not be ascribed to uneven distribution of T3 + cells, OKM1 + cells or B1 + (CD20) cells. However, the gpl50‐enriched population contained significantly more B2 + (CD21) cells than the gpl50‐depleted population. These results suggest that the gpl50 + B cells differ from gpl50‐B cells, not only in their responsiveness to T cell differentiation signals but also in their commitment to Ig heavy chain isotype secretion.