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Comparative Studies on Tree Pollen Allergens
Author(s) -
FLORVAAG E.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02275.x
Subject(s) - resolution (logic) , pollen , chemistry , biology , botany , computer science , artificial intelligence
Visualization by autoradiography of specific IgE binding in crossed radioimmunoelectrophoresis (CRIE) and other 125 I‐immunoautoradiography (IAR) techniques is done in two different ways; either by traditional direct autoradiography (D‐ARG), where the film is exposed to the 125 I‐anti‐lgE incubated sample at room temperature, or by indirect autoradiography (ID‐ARG), applying intensifying screen, low‐temperature exposure and, eventually, pre‐exposure. This study confirmed that D‐ARG provided the benefits of simplicity and better image resolution with the disadvantage of prolonged exposure periods. ID‐ARG reduced the exposures needed to produce film image densities of 0.01 and 0.1 A 540 nm (i.e. autoradiographic sensitivity (ARσ) and autoradiographic speed (ARs)) to 1/l8 and 1/55 respectively of the corresponding exposures in D‐ARG. The lowest detection limits for 125 I in 24 h were 1.2 cpm mm −2 with the indirect and 6.8cpm mm −2 with the direct systems investigated. The major drawbacks of ID‐ARG were inferior image resolution and higher background levels, especially when pre‐exposure was included.