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Immunohistochemical Detection of the Membrane and Fluid‐phase Terminal Complement Complexes C5b‐9(m) and SC5b‐9
Author(s) -
MOLLNES T. E.,
HARBOE M.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02270.x
Subject(s) - complement membrane attack complex , complement (music) , complement system , monoclonal antibody , terminal (telecommunication) , immunohistochemistry , membrane , antibody , lytic cycle , chemistry , complement component 5 , alternative complement pathway , classical complement pathway , microbiology and biotechnology , biology , immunology , biochemistry , computer science , phenotype , telecommunications , virus , complementation , gene
Activation of the terminal pathway of complement on a membrane results in the generation of the membrane‐damaging terminal C5b‐9(m) complement complex, whereas the non‐lytic water‐soluble SC5b‐9 complex is formed when complement is activated in the fluid phase. Both forms of the terminal complement complex (TCC) can be immunohistochemically detected. but not distinguished, by antibodies recognizing neoantigens in the complexes. By means of monoclonal antibodies against C9 neoantigens and against the S‐protein. it was demonstrated that deposits of the TCC in tissue sections may be either in the form of C5b‐9(m) or SC5b‐9. The consequences of this for the interpretation of the histochemical data and the terminology of the two complexes are discussed.