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Activation of Resting, Pure CD4 + , and CD8 + Cells via CD3
Author(s) -
HALVORSEN R.,
GAUDERNACK G.,
LEIVESTAD T.,
VARTDAL F.,
THORSBY E.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02252.x
Subject(s) - cd3 , microbiology and biotechnology , cd8 , monoclonal antibody , recombinant dna , biology , in vitro , chemistry , t cell , antibody , immunology , immune system , biochemistry , gene
We studied the requirements for secondary activation signals in pure CD4 + and CD8 + T cells after stimulation with anti‐CD3 antibodies. Stimuluion or CD4 + or CD8 + cells with anti‐CD3 monoclonal antibodies (MoAb) bound to polyptyene monosized particles never resulted in a proliferative response. However, DNA synthtsis was observed when recombinant interietikin 2 (IL‐2) or other secondary signals, such as those provided by phorbol myristate acetate (PMA) or autologous accessory cells (AC), were also added. These secondary signals were not in themselves capable of inducing DNA synthesis in the absence of particle‐bound anti‐CD3. We also found that the signals provided by AC may be dependent on the activation state of these cells. Thus, the effects of accessory cells were enhanceed by a factor present in fetal calf serum (FCS), must likely endotoxin or lipopolysaccharide (LPS), which alone, however, were not able to activate T cells, even in the presence of particle‐bound anti‐CD3. Recombinant lL‐1 over a broad dose range was unable to replace PMA or activated AC after slimulation with particle‐bound anti‐CD3. Purified CD4 + and CDS + T cells behaved idenlically in all the experiments, indicating that the basic mechanisms for activation in the two T‐cell subsets are idenlical.