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Effects of Ethanol on Human Monocyte IgG Fc Receptors
Author(s) -
MØRLAND B.,
MØRLAND J.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02250.x
Subject(s) - ethanol , phagocytosis , incubation , receptor , internalization , in vitro , rosette (schizont appearance) , chemistry , monocyte , antibody , andrology , immunology , endocrinology , biochemistry , biology , medicine
The IgG Fc receptor function of human monocytes (Mo) exposed to ethanol in vitro was assessed by a rosette assay in which IgG‐coated sheep red blood cells were used as test particles. Pre‐incubation of Mo in autologous serum with ethanol (55 mM and above) for 15 min at 37°C, caused a significantly lower percentage of Mo‐forming rosettes. This reduction by ethanol was not observed when Mo were preincubated with ethanol in fetal calf serum or in serum‐free conditions, but was present when Mo were pre‐incubated with ethanol in serum‐free media to which IgG (10‐150 μg/ml) had been added. Donor‐dependent differences were observed in the reduction of Mo‐forming rosettes in the presence of autologous serum and ethanol. Mo allowed to form rosettes without ethanol were exposed to ethanol during a subsequent phagocytosis process. A dose‐dependent inhibition of the internalization of test particles was found in Mo from all donors.