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Kinetics of Ca 2+ Uptake into Lectin‐Induced Secondary Lymphocytes during Reactivation with Concanavalin A or Interleukin 2
Author(s) -
WOLFF C. H. J.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02228.x
Subject(s) - concanavalin a , lectin , kinetics , extracellular , chemistry , microbiology and biotechnology , interleukin 2 , biophysics , dna , biology , lymphocyte , immunology , biochemistry , in vitro , physics , quantum mechanics
The kinetics of Ca 2+ uptake into lectin‐induced secondary lymphocytes was studied during reactivation to DNA synthesis with concanavalin A (Con A) or interleukin 2 (IL‐2). IL‐2 did not cause any significant uptake of Ca 2+ into the lymphocytes, while Con A induced an accumulation of Ca 2+ into the lymphocytes which reached a maximum 1 to 2h after the addition of the lectin. The time during which Ca 2+ uptake occurred corresponded to the time of dependence on extracellular Ca 2+ for lectin‐induced DNA synthesis. The increased rate of Ca 2+ accumulation and the shortened Ca 2+ dependence period of secondary lymphocytes as compared with primary lymphocytes could explain the ability of secondary lymphocytes to display an accelerated response, in terms of DNA synthesis, to re‐stimulus.