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Selective Inhibition of Human Natural Killing and Antibody‐Dependent Cellular Cytotoxicity by a Polyanion
Author(s) -
ROELLINGER S.,
LOOS M.,
LEIBOLD W.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02222.x
Subject(s) - antibody dependent cell mediated cytotoxicity , cytotoxicity , peripheral blood mononuclear cell , lysis , effector , antibody , cytotoxic t cell , interferon , biology , cell culture , microbiology and biotechnology , cell , natural killer cell , chemistry , immunology , in vitro , biochemistry , genetics
A high molecular polyanion, Liquoid. was found to inhibit at nontoxic concentrations (12–50μg/ml) the natural killing (NK) and ihe antibody‐dependent cellular cytotoxic (ADCC) activity of human peripheral blood mononuclear cells selectively. Whereas NK of the K 562 target cell was slightly or not at ail affected, the spontaneous lysis of PDe‐B‐1, an EB V‐transformed B‐cell line, was strongly inhibited or even completely abolished. ADCC activity could only be inhibited by Liquoid if the target cells were mycoplasma‐free, while the polyanion had no effect when mycoplasma‐contaminated target cells were used. Liquoid did noi alter the target binding capacity of the NK effector cells and did not activate monocytes or induce other suppressive cells. Alpha interferon, but neither beta nor gamma interferon, was able to neutralize ihe NK reduction. These results suggest that Liquoid inhibits a target cell‐related, selective process in the post‐binding stage of NK cell lysis.