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The Gene for the Leu‐M2 (Mac‐120) Antigen is Located on Human Chromosome 11
Author(s) -
SUOMALAINENNEVANLINNA H. A.,
SCHRÖDER J.,
ANDERSSON L. C.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02209.x
Subject(s) - antigen , microbiology and biotechnology , biology , cell sorting , flow cytometry , immunofluorescence , gene , chromosome , antibody , immunology , genetics
Leu‐M2 (Mac‐120) antigen is a cell‐surface marker present on a subpopulation of human blood monocytes and platelets. Its expression on monocytes and macrophages is necessary for their ability to present antigen to T lymphocytes. Only Leu‐M2+ cells display the human la‐like determinant, HLA‐DS, which seems important in antigen presentation and stimulation in the autologous MLR. Expression of the Leu‐M2/Mac‐120 antigen was studied by indirect immunofluorescence analysis and flow cytofluorometry in mouse‐human somatic cell hybrids segregating human chromosomes. In the mouse‐human hybrid clones, expression of the Leu‐M2/Mac‐120 antigen was dependent on the presence of human chromosome 11. This was verified by fluorescence‐activated cell sorting of heterogeneous hybrid cell populations into antigenpositive and ‐negative fractions. The Leu‐M2 antigen co‐segregated with chromosome 11. The gene controlling the expression of the Leu‐M2/Mac‐120 antigen thus is assigned to human chromosome 11.