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Cytotoxic T Lymphocytes: Blocked Differentiation at the ‘Poised’ Stage
Author(s) -
GROMO G.,
OCHOA A. C.,
BACH F. H.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb02204.x
Subject(s) - cytotoxic t cell , lytic cycle , cytotoxicity , microbiology and biotechnology , interleukin 2 , lymphokine , antigen , chemistry , in vitro , biology , immunology , biochemistry , virus
The effect of different concentrations of phorbol myristate acetate (PMA) on the development of cytotoxic cells was studied, PMA was selectively able to prevent the development of cytotoxic cells in a mixed leucocyte culture, while allowing the responding cells to proliferate. The higher concentration of PMA (10 −5 m ) blocked both direct cytotoxicity and lytic activity in the presence ot lectin, while the lower concentration (10 −8 m ) only prevented direct lytic function. The removal of PMA and subsequent addition of recombinant interleukin 2 (IL‐2) or IL‐2‐containing supernatants effectively reversed the effect of PMA with recovery of antigen‐specific lytic function of cells treated with 10 −8 m , while cells treated with 10 −5 m PMA only recovered lectin‐dependent cytotoxic ability.