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Human Alveolar Macrophages Synthesize Active Complement Components C6, C7, and C8 in Vitro
Author(s) -
PETTERSEN H. B.,
JOHNSON E.,
HETLAND G.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb01082.x
Subject(s) - in vitro , complement (music) , immunology , chemistry , microbiology and biotechnology , biology , biochemistry , phenotype , gene , complementation
We investigated whether serum‐free human alveolar macrophage cultures synthesize aetive C6. C7. and C8. There was a significant binding of polyclonal anti‐human C6 antibodies to agarose beads incubated with unstimulated macrophages for 24 or 48 h. Endotoxin stimulation of the macrophages was necessary for significant binding of polyclonal anti‐C7 and anti‐C8 antibodies to agarose beads co‐cultured for 48 or 96 h. Two monoclonal antibodies (poly C9‐MA and MCaE11) specific for a neoantigen of polymerized C9 in the terminal complement complex (TCC), bound to beads mainly incubated with endotoxin stimulated macrophages. The MCaEll was more sensitive than the poly C9‐MA in detecting the C9 neoantigen on beads incubated with the macrophages or human serum diluted 1:16. We thus conclude that human alveolar macrophages synthesize active C6, C7, and C9 that together with C5 and C9, assemble as the TCC on co‐cultured agarose beads. Activation of the alternative pathway on the agarose with generation of fixed C3 and C5 convertases is a prerequisite for the subsequent generation of the TCC.