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Enhancement and Suppression of an Intrastrain Cross‐Reactive Idiotype
Author(s) -
MANZO C.,
NISONOFF A.
Publication year - 1987
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1987.tb01065.x
Subject(s) - keyhole limpet hemocyanin , idiotype , immune system , monoclonal antibody , chemistry , antibody , hemocyanin , microbiology and biotechnology , biology , immunology
The effects of a copolymer of monoclonal anti‐idiotype (7B7.10) with keyhole limpet heamocyanin (KLH), designated 7‐K, on an ongoing immune response were investigated. It was found that the response could be diverted to the production of higher of antip‐azobenzenearsnate (Ar) antibodies, of which nearly 100% carry an intrastrain crossreactive idiotype (CRI A ). The effect was observed only in mice that had received a prreinoculation of KLH‐Ar, or KLH plus bovine gamma globulin‐Ar (BGG‐Ar). The effect was also observd, however, when cross‐linked 7B7.10 was mixed, rather than conjugated with KLH, suggesting that the role of KLH was to induce the production of a B‐cell growth factor. Cross‐linked 7B7.10 was not effective in the absence of KLH. A primary inculation of 7‐K together with KLH‐Ar did not result ini significant suppression or enhancement of CRI A . Also, pre‐inoculation of 7‐K alone did not suppress a subsequent idiotypic response to KLH‐Ar, whereas monomeric anti‐Id was suppressive. This supports a possible role for the unmodified Fc segment in the suppressive mechanism. In mice primed with KLH‐Ar, before administration of 7‐K, CRI A molecules lacking anti‐Ar activity were present in very low concentrations in the immune sera. The methods described permit the reproducible production of large amounts of CRI A anti‐Ar antibodies.